s aureus nctc 10656 Search Results


s aureus nctc 10656  (ATCC)
93
ATCC s aureus nctc 10656
S Aureus Nctc 10656, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+aureus+nctc+10656/Saitozyma+flava+(Saito)+Liu+et+al/pmc00254329-78-37-48
Average 93 stars, based on 1 article reviews
s aureus nctc 10656 - by Bioz Stars, 2026-09
93/100 stars
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denr  (Proteintech)
93
Proteintech denr
( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of <t>eIF4,</t> <t>eIF2α,</t> PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) <t>DENR-KD</t> (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .
Denr, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+aureus+nctc+10656/DENR+Antibody/pmc05309760-127-42-45
Average 93 stars, based on 1 article reviews
denr - by Bioz Stars, 2026-09
93/100 stars
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xsbr latex litex s 10656  (Synthomer Deutschland)
90
Synthomer Deutschland xsbr latex litex s 10656
( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of <t>eIF4,</t> <t>eIF2α,</t> PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) <t>DENR-KD</t> (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .
Xsbr Latex Litex S 10656, supplied by Synthomer Deutschland, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+aureus+nctc+10656/litextm+s+10656/us10836876-298-70-66
Average 90 stars, based on 1 article reviews
xsbr latex litex s 10656 - by Bioz Stars, 2026-09
90/100 stars
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sil glutathione  (Cambridge Isotope Laboratories)
93
Cambridge Isotope Laboratories sil glutathione
( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of <t>eIF4,</t> <t>eIF2α,</t> PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) <t>DENR-KD</t> (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .
Sil Glutathione, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+aureus+nctc+10656/N-Methylperfluorooctanesulfonamidoacetic+acid/pmc05788707-129-2-15
Average 93 stars, based on 1 article reviews
sil glutathione - by Bioz Stars, 2026-09
93/100 stars
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15 n]l-aspartic acid  (Cambridge Isotope Laboratories)
90
Cambridge Isotope Laboratories 15 n]l-aspartic acid
( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of <t>eIF4,</t> <t>eIF2α,</t> PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) <t>DENR-KD</t> (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .
15 N]L Aspartic Acid, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+aureus+nctc+10656/N-Methylperfluorooctanesulfonamidoacetic+acid/pmc06224545-421-44-46
Average 90 stars, based on 1 article reviews
15 n]l-aspartic acid - by Bioz Stars, 2026-09
90/100 stars
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n 1 methionine  (Cambridge Isotope Laboratories)
93
Cambridge Isotope Laboratories n 1 methionine
( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of <t>eIF4,</t> <t>eIF2α,</t> PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) <t>DENR-KD</t> (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .
N 1 Methionine, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+aureus+nctc+10656/N-Methylperfluorooctanesulfonamidoacetic+acid/pmc04132681-42-21-24
Average 93 stars, based on 1 article reviews
n 1 methionine - by Bioz Stars, 2026-09
93/100 stars
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pantothenic acid  (Cambridge Isotope Laboratories)
93
Cambridge Isotope Laboratories pantothenic acid
( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of <t>eIF4,</t> <t>eIF2α,</t> PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) <t>DENR-KD</t> (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .
Pantothenic Acid, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+aureus+nctc+10656/N-Methylperfluorooctanesulfonamidoacetic+acid/pmc04375488-102-41-52
Average 93 stars, based on 1 article reviews
pantothenic acid - by Bioz Stars, 2026-09
93/100 stars
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n 1 tyrosine  (Cambridge Isotope Laboratories)
93
Cambridge Isotope Laboratories n 1 tyrosine
( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of <t>eIF4,</t> <t>eIF2α,</t> PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) <t>DENR-KD</t> (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .
N 1 Tyrosine, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+aureus+nctc+10656/N-Methylperfluorooctanesulfonamidoacetic+acid/pmc03923163-188-11-46
Average 93 stars, based on 1 article reviews
n 1 tyrosine - by Bioz Stars, 2026-09
93/100 stars
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17 dsm106575  (DSMZ)
86
DSMZ 17 dsm106575
( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of <t>eIF4,</t> <t>eIF2α,</t> PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) <t>DENR-KD</t> (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .
17 Dsm106575, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+aureus+nctc+10656/Staphylococcus+pasteuri/pmc07807382-4666-4-21
Average 86 stars, based on 1 article reviews
17 dsm106575 - by Bioz Stars, 2026-09
86/100 stars
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n 1 trp  (Cambridge Isotope Laboratories)
93
Cambridge Isotope Laboratories n 1 trp
( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of <t>eIF4,</t> <t>eIF2α,</t> PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) <t>DENR-KD</t> (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .
N 1 Trp, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+aureus+nctc+10656/N-Methylperfluorooctanesulfonamidoacetic+acid/pmc00061027-359-8-13
Average 93 stars, based on 1 article reviews
n 1 trp - by Bioz Stars, 2026-09
93/100 stars
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n 1 pro  (Cambridge Isotope Laboratories)
93
Cambridge Isotope Laboratories n 1 pro
( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of <t>eIF4,</t> <t>eIF2α,</t> PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) <t>DENR-KD</t> (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .
N 1 Pro, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+aureus+nctc+10656/N-Methylperfluorooctanesulfonamidoacetic+acid/pmc04705402-52-55-58
Average 93 stars, based on 1 article reviews
n 1 pro - by Bioz Stars, 2026-09
93/100 stars
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n 1 serine  (Cambridge Isotope Laboratories)
93
Cambridge Isotope Laboratories n 1 serine
a,b,c The fractional labelling of serine, glycine ( a ), GSH, GSSG ( b ), and purine nucleotides (AMP, ATP, GMP, GTP) ( c ) from K562 cells treated for 24 h with 2 μM imatinib (IM), 2.5 μM SHIN1, or a combination of both in medium containing 140 μM 13 C 3 15 <t>N</t> <t>1</t> -serine. (n=4 independent wells from individual experiment). d,e, Main isotopologue(s) of serine, glycine ( d ) and GSH, GSSG ( e ) from CML CD34 + cells treated for 24 h with 2 μM imatinib (IM), 2.5 μM SHIN1, or a combination of both in medium containing 140 μM 13 C 3 15 <t>N</t> <t>1</t> -serine (n=4 patient samples). Isotopologues are plotted as the percentage of fraction of the sum of all isotopologues. Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Dunnett’s multiple comparisons test ( d,e ).
N 1 Serine, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of eIF4, eIF2α, PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) DENR-KD (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .

Journal: Scientific Reports

Article Title: A screening strategy for the discovery of drugs that reduce C/EBPβ-LIP translation with potential calorie restriction mimetic properties

doi: 10.1038/srep42603

Figure Lengend Snippet: ( a ) Mouse embryonic fibroblasts (MEFs) that are deficient in the mTORC1-inhibitor protein TSC1 (TSC1-KO) or wt MEFs transiently transfected with pcDNA3-Firefly Re-ini /Renilla Ini and pcDNA-Firefly Ref /Renilla Ref reporter plasmids. The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, TSC1, phospho-S6K, S6K and the β-actin loading control. ( b ) 4E-BP1/2 double knockout (DKO) MEFs and wt MEFs transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 5). Immunoblots show the levels of C/EBPβ, 4E-BP1, 4E-BP2 and the β-actin loading control. ( c ) HEK293T cells stably expressing pcDNA3-Firefly Re-ini /Renilla Ini or pcDNA-Firefly Ref /Renilla Ref reporters treated with 200 nM rapamycin or 10 nM PP242 for 8 h. The bar graphs show the calculated TRI mean values (n = 3). The immunoblots show mTORC1 inhibition by reduced phosphorylation of 4E-BP1 and S6K in addition to β-actin as loading control. ( d ) HEK293T cells described in ( c ) transfected with eIF expression vectors. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots show levels of eIF4, eIF2α, PKR and the β-actin loading control. ( e ) HEK293T cells described in ( c ) treated with the PKR inhibitor (C16) for 8 h. The bar graphs show the calculated TRI mean values (n = 3). Immunoblots shows the reduced phosphorylation of eIF2α and the β-actin loading control. ( f ) DENR-KD (+Dox) and DENR-Control (−Dox) HEK293T cells transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of DENR and the β-actin loading control. ( g ) C33A cells with stable SBDS knockdown (shSBDS) or control cells (shCTRL) transiently transfected with reporter constructs as described in ( a ). The bar graphs show the calculated TRI mean values (n = 6). Immunoblots show levels of SBDS and the β-actin loading control. Statistical differences were analyzed by Student’s t-tests. Error bars represent ± SD, *P < 0.05, **P < 0. 01, ***P < 0. 001. Full scans of the immunoblots are presented in .

Article Snippet: The following antibodies were used: C/EBPβ (C19) and SBDS (S-15) from Santa Cruz Biotechnology; phospho-p70S6K (Thr389) (108D2), p70S6K (#9202), Hamartin/TSC1 (D43E2) (#6935), phospho-4E-BP1 (Thr37/46) (#9459), 4E-BP1 (#9452), 4E-BP2 (#2845), eIF4E (#9742), Phospho-eIF2α (Ser51) (#9721), eIF2α (#9722) and PKR (#3072) from Cell Signaling; DENR (#10656-1-AP) from Proteintech TM and β-actin (clone C4) (#691001) from MP Biomedicals.

Techniques: Transfection, Western Blot, Control, Double Knockout, Construct, Stable Transfection, Expressing, Inhibition, Phospho-proteomics, Knockdown

a,b,c The fractional labelling of serine, glycine ( a ), GSH, GSSG ( b ), and purine nucleotides (AMP, ATP, GMP, GTP) ( c ) from K562 cells treated for 24 h with 2 μM imatinib (IM), 2.5 μM SHIN1, or a combination of both in medium containing 140 μM 13 C 3 15 N 1 -serine. (n=4 independent wells from individual experiment). d,e, Main isotopologue(s) of serine, glycine ( d ) and GSH, GSSG ( e ) from CML CD34 + cells treated for 24 h with 2 μM imatinib (IM), 2.5 μM SHIN1, or a combination of both in medium containing 140 μM 13 C 3 15 N 1 -serine (n=4 patient samples). Isotopologues are plotted as the percentage of fraction of the sum of all isotopologues. Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Dunnett’s multiple comparisons test ( d,e ).

Journal: bioRxiv

Article Title: Mitochondrial folate metabolism inhibition drives differentiation through mTORC1 mediated purine sensing

doi: 10.1101/2022.12.21.521404

Figure Lengend Snippet: a,b,c The fractional labelling of serine, glycine ( a ), GSH, GSSG ( b ), and purine nucleotides (AMP, ATP, GMP, GTP) ( c ) from K562 cells treated for 24 h with 2 μM imatinib (IM), 2.5 μM SHIN1, or a combination of both in medium containing 140 μM 13 C 3 15 N 1 -serine. (n=4 independent wells from individual experiment). d,e, Main isotopologue(s) of serine, glycine ( d ) and GSH, GSSG ( e ) from CML CD34 + cells treated for 24 h with 2 μM imatinib (IM), 2.5 μM SHIN1, or a combination of both in medium containing 140 μM 13 C 3 15 N 1 -serine (n=4 patient samples). Isotopologues are plotted as the percentage of fraction of the sum of all isotopologues. Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Dunnett’s multiple comparisons test ( d,e ).

Article Snippet: For tracing experiments, K562 cells and primary CML cells were cultured in Plasmax, a physiological cell culture medium , containing 140 μM 13 C 3 15 N 1 -serine (catalogue no. CNLM-474-H-PK; Cambridge Isotope Laboratories).

Techniques: